Gram Stain

The Gram stain is a fundamental part of the demonstration and classification of bacteria. Although it has been used for over a hundred years, its mechanism has been the subject of almost continuous disagreement. The disagreements revolve around the explanations as to why some bacteria retain the methyl violet and others do not; and what the relationship is between the dye, trapping agent, bacterial cell wall and bacterial cell contents.


Popescu, A., Doyle, RJ., (1996)
The Gram Stain after More than a Century
Biotechnic & Histochemistry, v.71, No.3, pp.145




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