Lillie's Cold Schiff Reagent
|Sodium metabisulphite||1.9 g|
|Hydrochloric acid, 0.15N||100 mL|
|Activated charcoal (powder)||0.5 g|
Dissolve the dye and metabisulphite in the hydrochloric acid at room temperature. Shake frequently, or place on a shaker for about 2 hours. The solution should be clear to pale yellow or light brown. Add the activated charcoal and shake for a minute or two. Filter into a graduated cylinder. Restore the volume to 100 mL with distilled water rinses of the residue. Store at 0-5°C in a tightly stoppered bottle.
Lillie, R.D., (1954)
Histopathologic technique and practical histochemistry Ed.2
Blakiston, New York, USA.