Lillie’s Cold Formulation
Solution
| Material | Amount | |
|---|---|---|
| Pararosanilin | 1 | g |
| Sodium metabisulfite | 1.9 | g |
| Hydrochloric acid, 0.15N | 100 | mL |
| Activated charcoal (powder) | 0.5 | g |
Preparation
- Dissolve the dye and metabisulfite in the hydrochloric acid at room temperature.
- Shake frequently, or place on a shaker for about 2 hours. The solution should be clear to pale yellow or light brown.
- Add the activated charcoal and shake for a minute or two.
- Filter into a graduated cylinder.
- Restore the volume to 100 mL with distilled water rinses of the residue.
- Store at 0-5°C in a tightly stoppered bottle.
Notes
- If a diluted Schiff reagent is needed, add a solution made of 1.9% sodium metabisulfite in 0.15N hydrochloric acid in any proportion to get any desired dilution.
- The solution should be colorless or very pale amber. Brown solutions will often stain poorly. Usually these have been made from a batch of basic fuchsin containing too little pararosanilin.
References
- Lillie, R.D., (1954)
Histopathologic technique and practical histochemistry Ed.2
Blakiston, New York, USA.
