Barger & de Lamater’s Formulation
Solution
| Material | Amount | |
|---|---|---|
| Pararosanilin | 1 | g |
| Distilled water | 400 | mL |
| Thionyl chloride | 1 | mL |
| Activated charcoal (powder) | 2 | g |
Preparation
- Dissolve the dye in the water with heat if needed.
- Add the thionyl chloride.
- Stopper well and store in the dark overnight.
- If not water clear, add activated charcoal and shake for about a minute.
- Filter.
- Store at 0-5°C in a tightly stoppered bottle.
Notes
- The solution should be colorless or very pale amber. Brown solutions will often stain poorly. Usually, these have been made from a batch of basic fuchsin containing too little pararosanilin.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Culling C.F.A., (1974)
Handbook of histopathological and histochemical techniques Ed. 3
Butterworth, London, UK.
