General Oversight stain
Also known as the Hematoxylin Erythrosine Saffron stain.
Dissolve the erythrosine B and filter.
Preserve with a few drops drops of chloroform.
|Tannic acid, 5% aqueous||1||mL|
Add the saffron stigmata to the water and heat in a boiling water bath for one hour. Filter, and add the formalin and tannic acid. Life is a few weeks.
No particular fixative was specified. 5µ paraffin sections of neutral buffered formalin fixed tissue are suitable but results may be improved by refixing in Bouin's fixative or saturated aqueous picric acid at 56°C for one hour prior to staining, then washing the sections in tap water to remove all yellow discolouration.
Biological Staining Methods, 6th ed. (1957)
Gurr, G. T.,
George T. Gurr, London, UK
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Last updated January 2019