Heidenhain’s Vanadium Hematoxylin

Ingredient Amount Function
Stock A
Hematoxylin 0.5 g Dye
Distilled water 100 mL Solvent
Stock B
Ammonium vanadate 0.25 g Mordant
Distilled water 100 mL Solvent
Working solution
Stock A 60 mL Dye
Stock B 30 mL Mordant


Compounding procedure
Make each stock solution seperately.
Combine in the specified proportions.


  1. Bring sections to water with xylene and ethanol.
  2. Place into the staining solution for an appropriate time.
  3. Rinse well with water.
  4. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected results


  1. The staining time should be determined by trial.


Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
    Böhm and Oppel, (1907)
    Manuel de technique microsopique, ed. 4, p. 105.
    Vigot, Paris, France




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