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Seidelin’s Iron Hematoxylin

Seidelin's Iron Hematoxylin

6
steps
5
materials

Materials

Solution A

MaterialAmountFunction
Ferric chloride0.6 gMordant
Distilled water95 mLSolvent
Hydrochloric acid0.75 mLAcidifier

Solution B

MaterialAmountFunction
Hematoxylin1 gDye
100% ethanol100 mLSolvent

Compounding Procedure

  1. Make each solution separately.
  2. For use, combine 3 parts solution A with 2 parts solution B.
  3. The working solution may be used immediately, but is not stable for long.

Protocol

  1. Bring sections to water with xylene and ethanol.
  2. Place into the staining solution for an appropriate time.
  3. Wash well in running tap water to blue.
  4. Rinse with distilled water.
  5. Counterstain if desired.
  6. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected Results

  • Nuclei  –  black
  • Background  –  as counterstain or unstained

Notes

  • The stock solutions are stable for some time.
  • The working solution should be made fresh.
  • This is a modification of Weigert’s iron hematoxylin.
  • The staining time should be determined by trial, but is likely about 10-20 minutes.

Safety Note

Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.

References

  1. Gray, Peter. (1954)
    The Microtomist’s Formulary and Guide.
    Originally published by: The Blakiston Co.
    Republished by: Robert E. Krieger Publishing Co.
    Citing:
    Seidelin, (1911). Parasitology, v. 4, p. 94.