Janssen's Iron Hematoxylin

Ingredient Amount Function
Hematoxylin 1 g Dye
Ferric ammonium sulphate 5 g Mordant
100% ethanol 5 mL Solvent
Distilled water 70 mL Solvent
Glycerol 15 mL Solvent
Methanol 15 mL Solvent


Compounding procedure
Dissolve the iron alum into the water.
Dissolve the hematoxylin into the ethanol.
Combine, and leave for one week at room temperature.
Filter, and then add the other ingredients.
Combines all solutions immediately they are dissolved,
and omits the week at room temperature.
The solution is stable for a few months.


  1. Bring sections to water with xylene and ethanol.
  2. Place into the staining solution for 15-30 minutes.
  3. Rinse with tap water.
  4. Dip briefly in 1% hydrochloric acid in 70% ethanol.
  5. Wash well in running tap water to blue.
  6. Rinse with distilled water.
  7. Counterstain if desired.
  8. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected results


  1. This solution may be used as an acid resistant nuclear stain.
  2. It is recommended as a substitute for Weigert's iron hematoxylin.


Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
    Janssen, (1897), Cellule, v.14, p.207.
    Lillie & Earle, (1939), Stain Technology, v.14, p.53



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