Faure's Iron Hematoxylin
Materials
Solution A
| Material | Amount | Function |
|---|---|---|
| Hematoxylin | 3.2 g | Dye |
| 90% ethanol | 100 mL | Solvent |
Solution B
| Material | Amount | Function |
|---|---|---|
| Ferric chloride | 0.2 g | Mordant |
| Cupric acetate | 0.1 g | Mordant |
| Distilled water | 100 mL | Solvent |
| Hydrochloric acid | 2 mL | Acidifier |
Compounding procedure
- Make each solution seperately.
- For use, combine equal parts of solutions A and B.
- The working solution may be used immediately, but is not stable for long.
Protocol
- Bring sections to water with xylene and ethanol.
- Place into the staining solution for 5 seconds.
- Rinse with tap water.
- Dip briefly in 1% hydrochloric acid in 70% ethanol.
- Wash well in running tap water to blue.
- Rinse with distilled water.
- Counterstain if desired.
- Dehydrate with ethanol, clear with xylene and mount with a resinous medium.
Expected Results
- Nuclei – black
- Background – as counterstain or unstained
Notes
- The stock solutions are stable for some time.
- The working solution should be made fresh.
- This method is suitable as an acid resistant nuclear stain.
- Only a very short staining time of 5 seconds is required.
- The solution incorporates two mordants, iron and copper.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Gray, Peter. (1954)
The Microtomist’s Formulary and Guide.
Originally published by: The Blakiston Co.
Republished by: Robert E. Krieger Publishing Co.
Citing:
Faure, (1929)
Compte rendu hebdomadaire des séances et mémoires de la Société de biologie,
v.90, p.87.
