Reddy's Alum Hematoxylin
Materials
| Material | Amount | Function |
|---|---|---|
| Hematoxylin | 6.4 g | Dye |
| Ammonium alum | 60 g | Mordant |
| Ethanol, absolute | 200 mL | Solvent |
| Glycerol | 160 mL | Stabiliser |
| Distilled water | 640 mL | Solvent |
Compounding Procedure
- Combine all the ingredients.
- Mix for a day.
- Leave in the dark for at least a month to ripen.
Protocol
- Bring sections to water with xylene and ethanol.
- Place into the staining solution for 3-5 minutes.
- Rinse with water.
- Differentiate lightly with 1% hydrochloric acid in 70% ethanol.
- Rinse with water.
- Blue with 0.1% sodium bicarbonate.
- Wash with water.
- Dehydrate with ethanol, clear with xylene and mount with a resinous medium.
Expected Results
- Nuclei – blue
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Reddy, P. H., (2001)
Neurological Sciences Institute
Oregon Health and Science University
