Hart's Stain
for Elastic Fibres


Weigert's iron hematoxylin or equivalent
Van Gieson's picro-fuchsin
Hart's solution
Weigert's iron resorcin fuchsin  –  1 volume
1% hydrochloric acid in 70% ethanol  –  9 volumes

Tissue sample
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.


  1. Bring sections to water via xylene and ethanol.
  2. Place into Hart's solution overnight.
  3. Wash with 95% ethanol to remove excess solution.
  4. Differentiate with 1% acid alcohol.
  5. Wash with tap water.
  6. Counterstain with iron hematoxylin and van Gieson.
  7. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected results


  1. This modification should be used if poor staining is obtained using Weigert's method.
  2. The optimum dilution may be more or less than given.


Culling C.F.A., (1974)
Handbook of histopathological and histochemical techniques Ed. 3
Butterworth, London, UK.




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