Dissolve the dye in the ethanol. Add paraldehyde and hydrochloric acid. Ripen at room temperature for 48-72 hours.
Refrigerate. The solution is stable for 2-3 months.
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable.
Other fixatives are likely to be satisfactory.
Bring sections to water via xylene and ethanol.
Wash with water.
Rinse with 70% ethanol.
Place in the staining solution for 10 minutes.
Rinse well with 95% ethanol.
Counterstain the nuclei and/or the cytoplasm if wished.
Dehydrate with ethanol, clear with xylene and mount with a resinous medium.
Elastic fibres – purple
Mast cells – purple
Pituitary β cells – purple
Sulphated mucins – purple
Background – as the counterstain
Nuclei – as the nuclear stain
The basic fuchsin used for this solution should be one that is suitable for Schiff's reagent,
i.e., it should have a high pararosanilin content. Both methods involve
forming a compound between an aldehyde and dye.