Klatskin's Modification
of Masson's trichrome for Liver


Harris' alum hematoxylin.
Solution A
Picric acid to saturation
Ethanol, 95% 100 mL
Solution B
Xylidine ponceau 1 g
Distilled water 99 mL
Glacial acetic acid 1 mL
Solution C
Phosphomolybdic acid 1 g
Distilled water 100 mL
Solution D
Aniline blue to saturation
Glacial acetic acid 2.5 mL
Distilled water 97.5 mL
Solution E
Acetic acid, glacial 1 mL
Distilled water 99 mL

Tissue sample
3-5 micron paraffin sections of formalin fixed liver.


  1. Bring sections to water via xylene and ethanol
  2. Stain with Harris' hemalum for 10 minutes.
  3. Rinse with 95% ethanol.
  4. Place in solution A for 10 minutes.
  5. Wash with water for 10 minutes.
  6. Place in solution B for 5 minutes.
  7. Place in solution C for 5 minutes.
  8. Place in solution D for 2 minutes.
  9. Replace in solution C for 5 minutes.
  10. Place in solution E for 5 minutes.
  11. Place in 70% ethanol for 2 minutes.
  12. Dehydrate with ethanol, clear and mount with a resinous medium.

Expected results


  1. Aniline blue, is a mixture of two dyes, methyl blue, and water blue. Either may usually be substituted satisfactorily.
  2. Although recommended for liver sections, this modification may also be used as a general replaceement for Masson's method.

Petersen, K.F., West, A.B., Reuben, A., Rothman, D. and Shulman, G.I. (1996)
Noninvasive Assessment of Hepatic Triglyceride Content in Humans With 13C Nuclear Magnetic Resonance Spectroscopy.
Hepatology, V. 24, p 114-117




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