5µ paraffin sections of neutral buffered formalin fixed tissue are suitable.
Other fixatives are likely to be satisfactory.
Most trichrome stains benefit from mercuric chloride fixation.
Treatment with picric acid may be less satisfactory due to the use of the basic dye, toluidine blue.
Bring sections to water via xylene and ethanol.
Stain nuclei with either Gallego's carbol fuchsin or Duprès' magenta
Wash with water.
Place into solution A for 10 minute.
Rinse with distilled water.
Place into solution B for 1-5 minutes.
Place into 95% ethanol until differentiated.
Dehydrate with 100% ethanol.
Clear with xylene and mount with a resinous medium
Nuclei – blue black (Gallego) or red (Duprès)
Erythrocytes – orange
Cytoplasm – red
Muscle – light green
Bone – dark green
Collagen – blue
The celestine blue-hemalum sequence or Weigert's iron hematoxylin may be suitable at step 2.
Reference Gray, Peter. (1954) The Microtomist's Formulary and Guide. Originally published by:– The Blakiston Co. Republished by:– Robert E. Krieger Publishing Co.
Citing:– Duprès, (1935) Monitore zoologico italiano, vol.46, pp.46 & 77