Cason's Trichrome
for Muscle and Collagen


Staining solution
Orange G 1 g
Acid fuchsin 1.5 g
Aniline blue 0.5 g
Phosphotungstic acid 1 g
Distilled water 100 mL

Tissue sample
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.


  1. Bring sections to water via xylene and ethanol.
  2. Place into the staining solution for 5 minute.
  3. Rinse with distilled water.
  4. Dehydrate with ethanol.
  5. Clear with xylene and mount with a resinous medium.

Expected results


  1. Although the method does not specifically call for one, an acid resistant nuclear stain could be inserted immediately before step 2.


Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
    Cason, (1950)
    Stain technology, vol.25, pp.225




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