Lillie's Allochrome


1% Aqueous periodic acid
Schiff's reagent
Weigert's iron hematoxylin
Picro-methyl blue
Saturated aqueous picric acid 100 mL
1% aqueous methyl blue 0.4 mL

Tissue sample
5 µ paraffin sections. Most fixatives, including 10% NBF, are satisfactory but those generally considered preferable for trichrome stains will be advantageous.


  1. Bring sections to water via xylene and ethanol
  2. Do a PAS stain, but do not counterstain
  3. Stain nuclei with Weigert's iron hematoxylin or equivalent.
  4. Wash well in tap water, rinse with distilled water.
  5. Place into picro-methyl blue for 6 minutes.
  6. Rinse well with 95% ethanol.
  7. Dehydrate with absolute ethanol.
  8. Clear with xylene and mount with a resinous medium.

Expected results

Histological demonstration techniques, (1974))
Cook, H C.
Butterworths, London, England




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