Protocol
Diazonium Reaction
for Enterochromaffin
Materials
- Mayer’s hemalum or similar
- Solution A
Material Amount Fast red B, 1% aqu. 5 mL Lithium carbonate, sat. aqu. 2 mL Refrigerate the stock solutions at 4°C. Use the working solution immediately it is prepared.
Tissue Sample
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable.
Protocol
- Bring sections to water via xylene and ethanol.
- Rinse with distilled water.
- Treat with solution A for 1 minute at 4°C.
- Rinse well with distilled water.
- Stain with Mayer’s hemalum for 1 minute.
- Wash well with running tap water.
- Dehydrate with ethanol, clear with xylene and mount with a resinous medium.
Expected Results
- Enterochromaffin – Orange-red
- Nuclei – Blue
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Bancroft, J. D. and Stevens, A. (1977).
Theory and practice of histological techniques.
Churchill Livingstone, Edinburgh, UK.
