Hughesdon's Metachromatic Stain
Paraffin sections of neutral buffered formalin fixed tissue are suitable. Mercuric chloride fixatives are reputed to emphasise metachromasia. Other fixatives may be satisfactory.
Acid mucopolysaccharides = Red/purple
Mast cells = Red/purple
Background = Blue
Drury, R A, and Wallington, E A, (1967).
Carleton's histological technique., Ed. 5. pp. 2250
Oxford University Press, London, England.
Last updated January 2019