Alizarin Red S for Calcium
|Alizarin red S||2||g||Ajust the pH to 4.2 with 10% ammonium hydroxide.|
|Distilled water||100||mL||Use a pH meter with a glass electrode.|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory. Fixatives containg acids may remove some calcium.
Bancroft, J.D. and Stevens A. (1982)
Theory and practice of histological techniques Ed. 2
Churchill Livingstone, Edinburgh & London, UK.
Last updated January 2019