Protocol
Burns, Pennock & Stoward's Thioflavine T
for Amyloid Fluorescence
Materials
- Krutsay’s hemalum
- Thioflavine T
Material Amount Thioflavine T 0.5 g Hydrochloric acid, 1N 100 mL
Tissue Sample
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable.
Protocol
- Bring sections to water via xylene and ethanol.
- Stain nuclei with alum hematoxylin.
- Rinse well with water.
- Stain with thioflavine T solution for 5 minutes.
- Blot, rinse well with absolute ethanol.
- Mount in a fluorescence free resinous mounting medium.
Expected Results
Using a UG1 or UG2 exciter filter and a UV barrier filter, or a BG12 exciter and an OG4 or OG5 barrier filter, amyloid fluoresces lime green or blue.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Burns, J., Pennock, C.A. and Stoward, P.J., (1967),
The specificity of the staining of amyloid deposits with thioflavine T,
Journal of pathology and bacteriology, v 94, page 337. - Bancroft, J.D. and Stevens A. (1982)
Theory and practice of histological techniques Ed. 2
Churchill Livingstone, Edinburgh & London, UK.
