Wolman's STB

Wolman's Standard Toluidine Blue for Amyloid


Toluidine blue solution
Toluidine blue 1 g
Distilled water 50 mL
Iso-propanol, absolute 50 mL

Tissue sample
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.


  1. Bring sections to water via xylene and ethanol.
  2. Place in the toluidine blue solution at 37°C for 30 minutes.
  3. Blot carefully.
  4. Place into absolute iso-propanol for one minute.
  5. Blot carefully.
  6. Clear with xylene and coverslip using Canada balsam.
  7. Examine microscopically using crossed polarising filters.

Expected results


  1. Wolman strongly recommended this procedure, considering it to be highly selective for amyloid.
  2. The birefringence is independent of section thickness and the quality of the microscope optics.


Wolman, M. (1971).
Amyloid, its nature and molecular structure: comparison of a new toluidine blue polarized light method with traditional procedures.
Laboratory Investigation, v. 25: p. 104-110.




Translate in
Google Translate