Duboscq-Brasil Fluid

Duboscq-Brasil fluid
Picric acid 1 g   
Ethanol, 80% 150 mL
Formalin, concentrated 60 mL
Acetic acid, glacial 15 mL
Duboscq-Brasil fluid – alternate formula
Picric acid, saturated alcoholic 12 mL
Ethanol, absolute 108 mL
Water 30 mL
Formalin, concentrated 60 mL
Acetic acid, glacial 15 mL

This solution is also known as Brasil's fluid, and Humason refers to it as "Bouin-Duboscq's" fluid. Since the solution is similar to alcoholic Bouin's fluid it should have similar characteristics, i.e. it is likely to be fairly rapid with some hardening, and promotion of staining with acid dyes. It may be recommended when glycogen is the target element. Both ethanol and picric acid enhance the preservation of glycogen either by trapping it within protein or by precipitation. Transfer directly to 95% ethanol.

Picric acid is explosive in the dry state, however it saturates at 8.96% in absolute ethanol. 11.2 mL of this solution contains fractionally over 1 gram of picric acid. The formula to the right, above, closely approximates the one to the left, but avoids weighing dry picric acid.


Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
    Brasil, (1904)
    Archives de zoologie expérimentale et générale,, vol. 2, p. 445

Shokeir, A. A., and Elbagoury, S. M.
Alloxan-Schiff reagent for the detection of fixed rabies virus in the nerve cell
J. med. Lab. Tech. (1966) vol. 23, p. 191

Humason, G.L., (1967)
Animal Tissue Techniques
W.H. Freeman & Co., San Francisco, CA, USA




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