Common Name: INT
Suggested Name: Iodonitrotetrazolium
Other Names:
–
C.I. Number: –
C.I. Name: –
Color: Red
Solubility Aqueous: Soluble
Solubility Ethanol: Soluble
Absorption Maximum: –
Empirical Formula: C19H13N5O2ICl
Formula Weight: 505.7
Description
Iodonitrotetrazolium chloride has been used to visualize dehydrogenase enzyme activity histochemically. As the enzyme removes hydrogen from the substrate it is transferred to a hydrogen acceptor, usually a co-enzyme. From the co-enzyme, it is transferred to the tetrazole, which is consequently reduced to its insoluble and intensely colored formazan. The formazan of INT is red.
References
- R. D. Lillie.
Conn’s Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A. - Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA. - Burstone, M.S.
Enzyme Histochemistry and its application to the study of neoplasms
Academic Press, New York, NY, USA
