Common Name: Pyronin B
Suggested Name: Pyronin B
Other Names:
–
C.I. Number: 45010
C.I. Name: –
Color: Red
Solubility Aqueous: Very
Solubility Ethanol: Very
Absorption Maximum: 550 (Gurr) 553 (Aldrich) 555 (Conn)
Empirical Formula: C21H27N2OCl
Formula Weight: 359 (Conn)1042.3 (Aldrich)
Description
The formula on the top is from Conn. The one on the bottom is from Green, and illustrates the suggestion that two molecules of pyronin B are linked through ferric chloride into a larger molecule due to oxidation with ferric chloride during manufacture. This explains the difference in formula weights.
Pyronin B is sometimes substituted for pyronin Y in the Unna-Pappenheim stain for nucleic acids, but is not generally considered to be as effective for that purpose.
Compare the formula with other pyronins.
References
- R. D. Lillie.
Conn’s Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A. - Susan Budavari, Editor,
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA - Green, F (1990)
The Sigma-Aldrich handbook of stains, dyes and indicators
Aldrich Chemical Company, Milwaukee, Wisconsin, USA - Edward Gurr, 1971
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.
