PEARSE' PAS Orange G
for Pituitary α & β cells
This method is also called the TRIPAS stain.
|Orange G||4||g||Dissolve the dye and acid in the water by shaking periodically for a few days, then leave completely undisturbed until the solution clarifies. Pipette off the clear solution and store in a tightly stoppered bottle. Do not filter to clarify the solution.|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable.
Mercuric chloride fixation was originally recommended.
Other fixatives are usually satisfactory, but glutaraldehyde should be avoided.
Lillie, R. D. and Fullmer, H.M., (1990)
Histopathologic technique and practical histochemistry, Ed. 4, pp. 278
Churchill Livingstone, London, England.
Bancroft, J. D. & Stevens, A., (1990)
Theory and practice of histological techniques, Ed. 3, pp. 345
McGraw-Hill Book Co., New York, USA.
Last updated January 2019