Naphthoic Acid Hydrazide
|Distilled water||to 100||mL|
|Veronal buffer pH7.4|
|Veronal acetate solution||5||mL|
|M/10 hydrochloric acid||5||mL|
|2-hydroxy-3-naphthoic acid hydrazide||0.1||g|
|Acetic acid, glacial||5||mL|
|Fast blue B|
|Fast blue B salt||0.1||g|
|Veronal acetate buffer (pH 7.4)||100||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Many other fixatives are satisfactory. Fixatives containing strong acids should be avoided if the intent is to demonstrate aldehydes generated from acid hydrolysis of DNA as acids in some fixatives may hydrolyse the tissue during fixation (picric acid in Bouin's formal-picric-acetic mixture for example).
Culling, C F A, Allison, R T, Barr, W T, (1985).
Cellular pathology technique., Ed. 4., p. 187
Butterworths, London, England.
Last updated July 2013.