Lillie's Schiff Reagent
|Distilled water||100 mL|
|Potassium metabisulphite||2 g|
|Hydrochloric acid, 1N||20 mL|
|Activated charcoal (powder)||0.3 g|
Bring the water to boiling. Remove from the heat and add the dye (be careful of foaming). Cool to 60° C and filter. Add the metabisulphite and the hydrochloric acid and mix well. Cover the opening and leave overnight or longer. Add the activated charcoal, shake well for about 1 minute and filter. Store at 0-5°C in a tightly stoppered bottle.
Lillie, R.D., (1954)
Histopathologic technique and practical histochemistry Ed.2
Blakiston, New York, USA.