Kasten and Burton’s Formulation
Solution
Material | Amount | |
---|---|---|
Pararosanilin | 50 | mg |
Distilled water | 300 | mL |
Sodium thiosulfate | 6 | g |
Preparation
- Dissolve the dye in distilled water.
- Add the sodium thiosulfate.
- The solution should decolorize immediately. Optionally, the solution may be boiled for one minute. The solution may be used immediately.
Notes
- The formula given in Humason specifies sodium hyposulfite. This is an older and obsolete name for sodium thiosulfate.
- The solution should be colorless or very pale amber. Brown solutions will often stain poorly. Usually these have been made from a batch of basic fuchsin containing too little pararosanilin.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Humason, G. L., (1967)
Animal tissue techniques, Ed. 2
W. H. Freeman and Company, San Francisco, Ca, USA