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Fluorescent Schiff Reagent

Fluorescent Formulation

Solution

MaterialAmount
Acriflavine1g
Distilled water200mL
Potassium metabisulfite2g
Hydrochloric acid, 1N20mL

Preparation

  1. Dissolve the dye in the water and mix well.
  2. Add the hydrochloric acid and mix well.
  3. Add the metabisulfite and mix well.
  4. Stopper tightly and leave 48 hours in the dark.
  5. Filter.

Notes

Safety Note

Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.

References

  1. Culling C.F.A., (1974)
    Handbook of histopathological and histochemical techniques Ed. 3
    Butterworth, London, UK.