Coleman’s Formulation
Solution
| Material | Amount | |
|---|---|---|
| Pararosanilin | 1 | g |
| Distilled water | 100 | mL |
| Potassium metabisulfite | 1 | g |
| Hydrochloric acid, 1N | 4 | mL |
| Activated charcoal (powder) | 0.25 | g |
Preparation
- Dissolve the dye into the water.
- Add the potassium metabisulfite.
- Add the acid and leave in the dark for 24 hours.
- Add the activated charcoal and shake for about a minute.
- Filter.
- Store at 0-5°C in a tightly stoppered bottle.
Notes
- Lillie used sodium metabisulfite instead of potassium metabisulfite.
- The solution should be colorless or very pale amber. Brown solutions will often stain poorly. Usually, these have been made from a batch of basic fuchsin containing too little pararosanilin.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Lillie, R.D., (1954)
Histopathologic technique and practical histochemistry Ed.2
Blakiston, New York, USA.
