Coleman's Schiff Reagent
|Distilled water||100 mL|
|Potassium metabisulphite||1 g|
|Hydrochloric acid, 1N||4 mL|
|Activated charcoal (powder)||0.25 g|
Dissolve the dye into the water. Add the Potassium metabisulphite. Add the acid, and leave in the dark for 24 hours. Add the activated charcoal and shake for about a minute. Filter. Store at 0-5°C in a tightly stoppered bottle.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Coleman, (1928). Stain technology, v. 13, p. 123.