Barger & de Lamater's Schiff Reagent
|Distilled water||400 mL|
|Thionyl chloride||1 mL|
|Activated charcoal (powder)||2 g|
Dissolve the dye in the water with heat if needed. Add the thionyl chloride. Stopper well and store in the dark overnight. If not water clear, add activated charcoal and shake for about a minute. Filter. Store at 0-5°C in a tightly stoppered bottle.
Culling C.F.A., (1974)
Handbook of histopathological and histochemical techniques Ed. 3
Butterworth, London, UK.