Llewellyn's Mordant Blue 3
Substitute for alum hematoxylin in the H&E stain
|An aqueous or alcoholic eosin Y solution|
|Mordant blue 3||0.25||g||1||g||1||g|
|Iron alum, 10% aqu.||40||mL||90||mL||5||mL|
|For all variants:|
|Dilute the iron alum with the water. Add the mordant blue 3 and dissolve. Add the specified acid, mix and filter The solutions are usable immediately and they have a shelf life of more than one year|
|Solution B||var A||var B|
|Hydrochloric acid, conc||1||mL||1||mL|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
|Click on a thumbnail to see a full sized image.|
Llewellyn, B. D., 1974
Mordant blue3: A readily available substitute for hematoxylin in the routine hematoxylin and eosin stain.
Stain Technology, v. 49, pp. 347.
Llewellyn, B. D., 1978
Improved nuclear staining with mordant blue 3 as a hematoxylin substitute
Stain Technology, v. 53, pp. 73.
Last updated January 2019