Kiernan's Eriochrome Cyanin
with two colours
|Eriochrome cyanine R
|Ferric chloride, 5.6%
|Sulphuric acid, conc.
||up to 500
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable.
Other fixatives are likely to be satisfactory.
- Bring sections to water via xylene and ethanol.
- Place sections into solution A for 3 minutes.
- Rinse with distilled water three times for about 20 seconds each time.
- Dehydrate with ethanols.
- Clear with xylene and mount with a resinous medium.
- Nuclei – purple blue
- Background – red
- Solution A is stable for years.
- Solution A must have a pH of 1.5 for the two colour effect. Check periodically, and adjust with 1M sulphuric or hydrochloric acids, or sodium hydroxide.
- Kiernan's also describes regressive nuclear staining and myelin staining variants.
- Eriochrome cyanine R is also known as mordant blue 3 and solochrome cyanine R
Kiernan. J.A., (1999)
Histological and histochemical methods: Theory and practice, Ed. 3
Butterworth Heinemann, Oxford, UK.
Last updated January 2019