Chick Stain
for Fungi
Materials
Solutions
- Weigert’s iron hematoxylin
- Acridine orange
Material Amount Acridine orange 0.1 g Distilled water 100 mL
Tissue Sample
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable.
Protocol
- Bring sections to water via xylene and ethanol.
- Place in Weigert’s iron hematoxylin for 5 minutes.
- Wash well with tap water.
- Place in acridine orange solution for 2 minutes.
- Wash in tap water for 30 seconds.
- Dehydrate, clear and mount in a non fluorescent resinous medium.
Expected Results
Using a BG 12 exciter filter, and OG 4 (yellow) and/or OG5 (orange) barrier filter, fungi fluoresce green-red.
Notes
- This is most suitable as a screening method.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Culling C.F.A., (1974)
Handbook of histopathological and histochemical techniques Ed. 3
Butterworth, London, UK.
