Seidelin's Iron Hematoxylin
Materials
Solution A
Material | Amount | Function |
---|---|---|
Ferric chloride | 0.6 g | Mordant |
Distilled water | 95 mL | Solvent |
Hydrochloric acid | 0.75 mL | Acidifier |
Solution B
Material | Amount | Function |
---|---|---|
Hematoxylin | 1 g | Dye |
100% ethanol | 100 mL | Solvent |
Compounding Procedure
- Make each solution separately.
- For use, combine 3 parts solution A with 2 parts solution B.
- The working solution may be used immediately, but is not stable for long.
Protocol
- Bring sections to water with xylene and ethanol.
- Place into the staining solution for an appropriate time.
- Wash well in running tap water to blue.
- Rinse with distilled water.
- Counterstain if desired.
- Dehydrate with ethanol, clear with xylene and mount with a resinous medium.
Expected Results
- Nuclei – black
- Background – as counterstain or unstained
Notes
- The stock solutions are stable for some time.
- The working solution should be made fresh.
- This is a modification of Weigert’s iron hematoxylin.
- The staining time should be determined by trial, but is likely about 10-20 minutes.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Gray, Peter. (1954)
The Microtomist’s Formulary and Guide.
Originally published by: The Blakiston Co.
Republished by: Robert E. Krieger Publishing Co.
Citing:
Seidelin, (1911). Parasitology, v. 4, p. 94.