Morel & Bassal's Iron Hematoxylin
Materials
Solution A
| Material | Amount | Function |
|---|---|---|
| Hematoxylin | 1 g | Dye |
| 95% ethanol | mL | Solvent |
Solution B
| Material | Amount | Function |
|---|---|---|
| Ferric chloride | 2 g | Mordant |
| Cupric acetate | 0.04 g | Mordant |
| Distilled water | 100 mL | Solvent |
| Hydrochloric acid | 1 mL | Acidifier |
Compounding Procedure
- Make each solution separately.
- For use, combine equal parts of solutions A and B.
- The working solution may be used immediately, but is not stable for long.
Protocol
- Bring sections to water with xylene and ethanol.
- Place into the staining solution for an appropriate time.
- Rinse with tap water.
- Dip briefly in 1% hydrochloric acid in 70% ethanol.
- Wash well in running tap water to blue.
- Rinse with distilled water.
- Counterstain if desired.
- Dehydrate with ethanol, clear with xylene and mount with a resinous medium.
Expected Results
- Nuclei – black
- Background – as counterstain or unstained
Notes
- The stock solutions are stable for some time.
- The working solution should be made fresh, and is likely not stable for long.
- The staining time should be determined by trial.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Gray, Peter. (1954)
The Microtomist’s Formulary and Guide.
Originally published by: The Blakiston Co.
Republished by: Robert E. Krieger Publishing Co.
Citing:
Morel and Bassal, (1909)
Journal de l’anatomie et de la physiologie normales et pathologique de l’homme
et des animaux, v. 45, p. 632.
