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Crétin’s Iron Hematoxylin

Crétin's Iron Hematoxylin

8
steps
6
materials

Materials

Solution A

MaterialAmountFunction
Ferrous sulfate4 gMordant
Distilled water100 mLSolvent

Solution B

MaterialAmountFunction
Potassium ferrocyanide2 gMordant
Potassium ferricyanide1 gMordant
Distilled water100 mLSolvent

Solution C

MaterialAmountFunction
Hematoxylin0.5 gDye
Distilled water100 mLSolvent

Solution D

MaterialAmountFunction
Ferric ammonium sulfate5 gMordant
Distilled water100 mLSolvent

Compounding procedure

Make each solution seperately.

Protocol

  1. Bring sections to water with xylene and ethanol.
  2. Place into solution A for 24 hours.
  3. Wash with running tap water overnight.
  4. Place into solution B for 3-6 hours.
  5. Rinse with distilled water.
  6. Place into solution C overnight.
  7. Place into solution D until differentiated.
  8. Dehydrate with ethanol, clear with xylene and mount with a resinous medium.

Expected Results

  • Nuclei – dense black

Safety Note

Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.

References

  1. Gray, Peter. (1954)
    The Microtomist’s Formulary and Guide.
    Originally published by: The Blakiston Co.
    Republished by: Robert E. Krieger Publishing Co.