Debiden's Alum Hematoxylin Variants
|Hematoxylin||5 g||5 g||Dye|
|Potassium alum||100 g||100 g||Mordant|
|Distilled water||1 L||1 L||Solvent|
|Glacial acetic acid||20 mL||20 mL||Acidifier|
|Mercuric oxide, red||2.5 g||–||Oxidant|
|Javex (5.25% sodium hypochlorite)||–||2 mL||Oxidant|
Compounding procedure (Both)
Dissolve the Alum in the water in a large flask.
Add the hematoxylin. Mix well.
Place in a 60-65°C oven overnight.
Remove from the oven, and add the appropriate oxidising agent.
Stir until cold. Filter.
Acetic acid may be added as soon as it is cooled following preparation.
It is usable immediately.
It is preferable to prepare small volumes monthly.
This improves in staining ability if allowed to ripen for a week or two.
If acetic acid is used it should be added just before use.
Debiden, D., (1987)
Improved preparation of Harris' hematoxylin
Histologic, v.18, No.8
Debiden, D., (1991)
A new oxidant for Harris' hematoxylin
Histologic, v.21, No.2