[email protected];s Trichrome
for Muscle, Cartilage and Collagen
|A red nuclear stain|
|Aniline blue||0.06||g||Add both dyes to the water.|
|Orange G||0.2||g||Bring to the boil for three minutes.|
|Phosphomolybdic acid||1||g||Add the phosphomolybdic acid while hot.|
|Distilled water||100||mL||Cool and filter.|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory. Most trichrome stains benefit from picric acid or mercuric chloride fixation. Formalin fixed tissues may benefit from secondary fixation of sections in Bouin's fluid.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Zeitschrift für wissenschaftliche Mikroskopie und für mikroskopische Technik,
v. 49, pp. 337
Last updated January 2019