for Fetal & Endocrine Tissue
|Hematoxylin, 10% in 95% ethanol||16||mL|
|Ammonium ferric sulphate||24||g|
|Acetic acid, glacial||1.2||mL|
|Working Solution A|
|Make each A solution ensuring all solids are dissolved.|
|Combine solutions A-1 and A-2 and mix well.|
|Add solution A-3 to the combined solution.|
|Aniline blue or Methyl blue||3||g|
|Acetic acid, glacial||1.5||mL|
|Picric acid, sat. aqueous||150||mL|
5µ paraffin sections of Bouin’s picric-acetic-formalin fixed fetal tissue, or endocrine tissue fixed in the Bouin’s mixture, but without the acetic acid.
Dawes, R. L. F., (1972),
A simple connective tissue stain suitable for foetuses and endocrine organs.
Medical Laboratory Technology, v. 29, Nº 1, p.27.
Last updated January 2019