Sandifords Stain
for Plasma Cells
Materials
Staining solution
| Material | Amount | |
|---|---|---|
| Distilled water | 75 | mL |
| Glycerol | 20 | mL |
| Ethanol, 95% | 5 | mL |
| Methyl green | 0.15 | g |
| Pyronin Y | 0.5 | g |
| Phenol | 1.5 | g |
Tissue Sample
Most fixatives should be satisfactory if fixation is not extended. Reagents which cause depolymerisation of DNA should be avoided. Decalcification may interfere with staining.
Protocol
- Bring sections to water via xylene and ethanol.
- Place into the staining solution for 5-10 minutes.
- Rinse briefly with water.
- Dehydrate rapidly with acetone.
- Clear with xylene and mount with a resinous medium.
Expected Results
- Nuclei – violet
- Plasma cell cytoplasm – red
- Other cell cytoplasm – pink
Notes
- The reference gave no details of the method to use. The details above are from that given for Pappenheim’s solution, and should be suitable as a starting point.
- Time and temperature of staining may need to be increased.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Gray, Peter. (1954)
The Microtomist’s Formulary and Guide. p. 355
Originally published by: The Blakiston Co.
Republished by: Robert E. Krieger Publishing Co.
