Paneth cells are located at the base of the glands of the small intestine and contain numerous cytoplasmic, acidophilic granules. For that reason, they can be seen in an H&E stain well enough for identification, and their demonstration with selective staining methods is rarely requested. They are often used for staining practice by those learning histological techniques due to their easy availability and because their demonstration uses an early "yellowsolve" staining method: Lendrum's phloxine-tartrazine. The alkaline sirius red method also demonstrates them well. It is obvious that the methods for their demonstration are very similar to those for eosinophils, both being cells containing strongly acidophil granules.
|Llewellyn's sirius red|
|Paneth cell||Intestinal gland|
Last updated January 2019