for Mast Cells
|Ferric ammonium sulphate||0.48||g|
|Walpole's acetate HCl buffer||100||mL|
|Walpole's acetate-HCl buffer, pH 1.42|
|Sodium acetate, M/1||100||mL|
|Hydrochloric acid, M/1||120||mL|
Paraffin sections of neutral buffered formalin fixed tissue are suitable. Mercuric chloride fixatives are reputed to emphasise metachromasia. Other fixatives may be satisfactory.
Culling, C F A, (1974).
Handbook of Histopathological and Histochemical Techniques., Ed. 3. pp. 169 & 419
Butterworths, London, England.
Last updated January 2019