As well as his nuclear staining hemalum variants, Mayer also published an alum hematein solution to demonstrate mucin, termed Mucihematein. This method is now obsolete, but was designed to accentuate the bluish colour of mucins seen with many strong, regressive hemalum formulations.
|Ingredient||Var I||Var II||Var III||Function|
|Hematein||0.2 g||1 g||0.2 g||Dye|
|Aluminum chloride||0.1 g||0.5 g||0.1 g||Mordant|
|Distilled water||60 mL||Solvent|
|Ethanol, 70%||100 mL||100 mL||Solvent|
|Nitric acid||drops||0.05 mL||pH control|
Grind the hematein with a few drops of the glycerol. Add the aluminum chloride, and mix. Add the glycerol and water, and mix. Test the solution on a positive control (e.g. stomach).
Make the 70% ethanol from absolute ethanol and tap water. Dissolve the hematein and aluminum chloride in it, and leave for one week. Test the solution on a positive control (e.g. stomach). If too pale add 1 drop of fresh 1% nitric acid and retest. Repeat if necessary, but no more than about 3 drops should be added.
Make the 70% ethanol from 95% ethanol and water. Dissolve the hematein and aluminum chloride in it. Add the nitric acid. Test the solution on a positive control (e.g. stomach).
Bensley R. R. and Bensley, S. H., (1938)
Handbook of Histological and Cytological Technique.
U. Chicago Press, Chicago, USA
Bolles Lee, A.. Edited by Gatenby, J.B. and Beams, H.W., (1950)
The Microtomist's Vade-Mecum. 11 ed.,
Churchill, London, UK.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Mitteilungen aus der Zoologischen Station zu Neapel, v. 12, p. 303
Mallory, F. B. & Wright, J.H., (1904)
Pathological technique, Ed.3
W. B. Saunders, Philadelphia, USA.