Best's Carmine For Glycogen
|Combine the water, potassium carbonate and carmine. Boil for 15 minutes. Cool and filter. Add the ammonia.|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. It is often recommended that an alcoholic fixative be used to preserve glycogen, but the majority may be demonstrated following formalin fixation. Alcohol fixation is not usually required except for critical applications.
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
Archiv für mikroskopische Anatomie, v. 23, p. 520
Bancroft, J. D. and Stevens, A., (1977)
Theory and practice of histological techniques
Churchill Livingstone, Edinburgh, UK
Last updated January 2019