Llewellyn's Sirius Red
|Alkaline sirius red|
|Sirius red F3B||0.5||g|
|Dissolve the dye into the water, add ethanol and mix well. Add 1 mL of 1% sodium hydroxide. Then, while strong backlighting and swirling, add drops of 20% sodium chloride until a fine haze is detected. Usually about 2 mL is adequate. Adding more than 4 mL causes excessive precipitation. The solution is reasonably stable for several months, but slowly deteriorates. Extend the staining time to compensate. When it requires more than 2 hours to adequately stain, prepare a new solution.|
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable. Other fixatives are likely to be satisfactory.
Llewellyn, B.D., (1970)
An improved sirius red method for amyloid.
Journal of Medical Laboratory Technology, v 23, 308
Last updated January 2019