King's Silver Impregnation
|Ethanol 50%, 75%, 95%.|
|Sodium carbonate, anhydrous||3.5||g|
|Silver nitrate, 10%|
|Silver nitrate, 10% aqueous||5||mL|
|Strong ammonia (S.G. 0.880)||as||required|
|Sodium carbonate, aqueous||6.8||mL|
Add ammonium hydroxide drop by drop to 5 mL of the silver solution until the precipitate which forms just redissolves. Add 6.8 mL sodium carbonate solution and sufficient water to make up to 75 mL. For use, add a few drops of pyridine to each 10 mL of the solution.
10-15µ free floating frozen sections of formalin fixed tissues. Collect into water and wash in a few changes of distilled water to remove residual formalin.
King, L.S., (1948),
Atypical Amyloid Disease: With Observation on a New Silver Stain for Amyloid,
American Journal of Pathology, v 24, page 1095-1115