Solutions Crystal violet, 1% aqueous
Acetic acid, 1% aqueous Methyl green, 2% aqueous, washed with chloroform to remove crystal violet
5µ paraffin sections of neutral buffered formalin fixed tissue are suitable.
Other fixatives are likely to be satisfactory. Cryostat sections usually show brighter metachromasia.
Unmounted frozen sections may also be floated in each solution and mounted on a slide just before coverslipping.
Bring sections to water via xylene and ethanol, except for cryostat and frozen sections.