Lüko’s Fluid
Tellyesniczky’s Fluid, Formal Alcohol Acetic
Formula
| Material | Amount | |
|---|---|---|
| Ethanol, 70% | 1 | L |
| Formalin, concentrated | 100 | mL |
| Acetic acid, glacial | 50 | mL |
Description
This fixative is usually referred to as Tellyesniczky’s fluid but, according to Gray, Tellyesniczky himself ascribed it to his assistant, Bela V. Lüko. It is also commonly known as formal alcohol acetic.
It is sometimes recommended for tissues in which it is important to preserve glycogen. High ethanol concentration fluids are often considered to be preferable for this purpose.
Time
Up to 24 hours.
Aftertreatment
Transfer to ethanol with a concentration higher than 70%.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Gray, Peter. (1954)
The Microtomist’s Formulary and Guide.
Originally published by: The Blakiston Co.
Republished by: Robert E. Krieger Publishing Co.
Citing:
Ehrlich, P., Krause, R., Mosse, M., Rosin, H and Weigert, K. (1910)
Enzyklopädie der mikroskopischen Teknik, ed. 2, vol. 2.
Urban und Schwartenberg, Berlin. - Humason,G. (1962 and 1972).
Animal Tissue Techniques.
W.H.Freeman and Co., San Francisco.
