Water 250 mL
Mercuric chloride 3 g
Chromium trioxide 1.8 g
Potassium dichromate 1.2 g
Sodium sulphate 0.6 g
Acetic acid, glacial 12 mL

No compounding instructions are given. A suggestion is to dissolve each ingredient completely in the order given.

No information is given as to fixation time and quality. However, the presence of mercuric chloride, chromium trioxide and potassium dichromate indicates that fixation should be fairly rapid, perhaps with some avidity for acid dyes. Nuclear staining may be diminished due to the chromium trioxide content.

Ruffini also suggested a modification to this fixative containing 3 mL acetic acid instead of 12.


Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
    Ruffini, A., (1927)
    Processidi technica embriologica ed istologica
    Licinio Cappelli, Bologna




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