|Acetic acid, glacial||70||mL|
|Mix equal parts of solutions A and B just before use.|
Randolph's fluid is a modification of Navishin's fluid and is somewhat weaker, but more convenient to prepare. It is used for the same purpose, i.e. chromosomes. It is sometimes referred to as "Craf" or "CRAF", both of which refer to "chromium, acetic, formalin", the three active ingredients.
Fixation of average sized pieces is complete after 12 to 24 hours, and then they must be washed very well with running tap water to remove all traces of chromium trioxide.
Davenport, H.A., (1960),
Histological and histochemical techniques,
W. B. Saunders Co., London, UK., Oxford, UK.