Randolph’s Fluid
Formula
Solution A
Material | Amount | |
---|---|---|
Chromium trioxide | 10 | g |
Acetic acid, glacial | 70 | mL |
Distilled water | 930 | mL |
Solution B
Material | Amount | |
---|---|---|
Strong formalin | 300 | mL |
Distilled water | 700 | mL |
Mix equal parts of solutions A and B just before use.
Description
Randolph’s fluid is a modification of Navishin’s fluid and is somewhat weaker, but more convenient to prepare. It is used for the same purpose, i.e. chromosomes. It is sometimes referred to as “Craf” or “CRAF”, both of which refer to “chromium, acetic, formalin”, the three active ingredients.
Fixation of average sized pieces is complete after 12 to 24 hours, and then they must be washed very well with running tap water to remove all traces of chromium trioxide.
Safety Note
Prior to handling any chemical, consult the Safety Data Sheet (SDS) for proper handling and safety precautions.
References
- Davenport, H.A., (1960),
Histological and histochemical techniques,
W. B. Saunders Co., London, UK., Oxford, UK.